Coupling LC with On - line ESI and Off - line MALDI – MS / MS for Maximum Information Readout from Protein Samples
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چکیده
were re-crystallized with ethanol/acetone/0.1%TFA (60:30:10, v:v). MS and MS/MS spectra were obtained fully automatically on an ultraflex MALDI–TOF/TOF mass spectrometer (Bruker Daltonics). The WARP–LCTM software controlled the intelligent precursor ion selection and BioTools 3.0 was used for ESI and MALDI spectra validation (both Bruker Daltonics). Database search: Mascot Version 2.0.05 (www. matrixscience.com) with MSDB was used. Only peptides with individual ion scores above the identity threshold (p < 0.05) were taken into account to determine identified proteins. Results were manually verified with WARP-LCTM.
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تاریخ انتشار 2005